> Eight-week-old mice were used for the spike protein injections. S1 protein was purchased from Acrobiosystems (Cat #S1N-C52H4, Newark, DE, USA), and used after polymixin B (Cat #P1004, Sigma-Aldrich, St. Louis, MO, USA) treatment (30 µg/ml). The animals were anesthetized with isoflurane, and the injection paths were drilled into their skulls. Then, 5 µg of S1 protein (1 µg/µl) were bilaterally injected into each hippocampal region using a Hamilton syringe (Cat #80330, Hamilton Company, Reno, NV, USA) attached to a syringe pump (Cat #53311, Stoelting Co., Wood Dale, IL, USA) at a constant volume of 0.5 µl/min.
I think we can all agree that injecting huge amounts of viral protein into your brain is not recommended.
This study did not include a control group which received non-spike protein viral antigens (from say influenza which is also known to cause neurocognitive problems). As such it does not prove any specific neurotoxic effect of Covid19. The mechanistic studies in the paper suggest it is non-specific inflammation which causes neuronal cell death.
I think we can all agree that injecting huge amounts of viral protein into your brain is not recommended.
This study did not include a control group which received non-spike protein viral antigens (from say influenza which is also known to cause neurocognitive problems). As such it does not prove any specific neurotoxic effect of Covid19. The mechanistic studies in the paper suggest it is non-specific inflammation which causes neuronal cell death.